Narsingh Yadav's hope of competing Rio were dashed with the Court of Arbitration for Sport (CAS) upholding the World Anti Doping Agency's (WADA) appeal against the clean chit given to him in a doping violation case.
It also slapped a four-year ban on the Mumbai wrestler.
"It is very sad and unfortunate. We were hopeful until the last proceeding of the CAS that he will get through which unfortunately did not happen," Indian Chef de Mission Rakesh Gupta told news agency IANS from Rio de Janeiro after the hearing.
"It is really very sad because he had a real potential to clinch a medal."
"WADA filed an urgent application before the CAS ad hoc division to challenge the decision of NADA India to exonerate Narsingh Yadav following two positive anti-doping tests with methandienone on June 25 and July 5. The athlete asserted that he was the victim of sabotage (food/drink tampering) by another person. WADA requested that a four-year period of ineligibility be imposed on the athlete," the CAS said in a statement.
"The CAS panel did not accept the argument of the athlete that he was the victim of sabotage and noted that there was no evidence that he bore no fault, nor that the anti-doping rule violation was not intentional. Therefore the standard four-year period of ineligibility was imposed by the panel," the statement added.
Narsingh was entangled in a doping controversy days before the Olympics, but was cleared by the NADA ahead of the Games.
However, WADA appealed to the CAS against the NADA's clearance to Narsingh just two days before he was scheduled to compete at the Games.
"The CAS panel in charge of this matter heard the parties and their representatives today. The parties were informed that the application was upheld, that Narsingh Yadav was sanctioned with a four-year ineligibility period starting today and that any period of provisional suspension or ineligibility effectively served by the athlete before the entry into force of this award shall be credited against the total period of ineligibility to be served," the CAS stated.